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Antibody(protein degradation)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(protein degradation)

【A】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ATF62011-08-29 70B1413.1WBIF IHCIP
Lifespan
mouse
Mono, IgG1,k

Hela細胞
ATF6(human)2016-07-11 WBIF IHCIP
BioAcademia1/10001-3ug
73-500
Mouse
IgG2a κ

HeLa, HEK293
ATF6(human)2018-07-12 WBIF IHCIP
BioAcademia1/10001-3ug
73-500
Mouse
IgG2a κ

HeLa, HEK293
ATF6(human/mouse)2016-07-11 WBIF IHCIP
BioAcademia1/1000
73-505
Mouse
IgG2a κ

HeLa, HEK293
ATF6(human/mouse)2018-07-12 WBIF IHCIP
BioAcademia1/1000
73-505
Mouse
IgG2a κ

HeLa, HEK293
AMFR/gp782014-06-19 WBIF IHCIP
Cell Signalingx1000
9590
Rabbit
IgG1

HeLa

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Cathepsin D2011-08-29 Ab-2WBIF IHCIP
CALBIOCHEMx20
rabbit
【PFA+Triton】:×,【PFA+Saponin】:◎,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
Lamp1, LBPAと共局在する。
Colocalize with lamp1 and LBPA.
Hela細胞
Cathepsin D2011-08-29 WBIF IHCIP
CALBIOCHEMx2000
rabbit
【PFA+Triton】:×,【PFA+Saponin】:◎,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
Lamp1, LBPAと共局在する。
Colocalize with lamp1 and LBPA.
Hela細胞
Calnexin2014-06-19 C5C9WBIF IHCIP
Cell Signalingx1000
2679
Rabbit
IgG1

HeLa
mouse cathepsin D2011-08-10 WBIF IHCIP
R&D Systemsx100
AF1029
3%PFA (in PBS)
goat

マウスMEF
CathepsinD2011-09-27 WBIF IHCIP
Biodesign1:10001:500
K5016R
rabbit
p

HeLa, A549, MCF7他

【D】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Derlin-22018-07-26 WBIF IHCIP
Sigma1/5001-3ug
D1194
Rabbit

HCT116
Derlin-12018-07-26 WBIF IHCIP
MBL1/10001-3ug
PM018
Rabbit

HCT116, DT40
Derlin-22018-07-26 WBIF IHCIP
MBL1/5001-3ug
PM019
Rabbit

HCT116, DT40
Derlin-12018-07-26 WBIF IHCIP
Sigma1/1000
SAB4200148
Mouse

HCT116

【E】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
EDEM32016-07-11 WBIF IHCIP
Sigma1/1000
E0409
Mouse

HCT116, HEK293, HeLa, HepG2
EDEM32018-06-20 WBIF IHCIP
Sigma1/1000
E0409
Mouse

HCT116, HEK293, HeLa, HepG2
EDEM12016-07-11 WBIF IHCIP
Sigma1/500
E8406
Rabbit

HCT116, HEK293, HeLa, HepG2
EDEM12018-06-20 WBIF IHCIP
Sigma1/500
E8406
Rabbit

HCT116, HEK293, HeLa, HepG2
EDEM32016-07-11 WBIF IHCIP
Sigma1/1000
E8906
Rabbit

HCT116, HEK293, HeLa, HepG2
EDEM32018-06-20 WBIF IHCIP
Sigma1/1000
E8906
Rabbit

HCT116, HEK293, HeLa, HepG2
EDEM22016-07-11 WBIF IHCIP
Sigma1/500
E9906
Rabbit

HCT116, HEK293, HeLa, HepG2
EDEM22018-06-20 WBIF IHCIP
Sigma1/500
E9906
Rabbit

HCT116, HEK293, HeLa, HepG2
EDEM22018-07-12 WBIF IHCIP
Novus1/1000
NBP2-37921
Rabbit
IgG

HCT116, HEK293

【H】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Hrs-22011-08-29 A-5WBIF IHCIP
ALEXISx50
mouse
【PFA+Triton】:×,【MeOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG2a
MeOH固定
Fixation by MeOH is best.
Hela細胞
HRD1/SYVN12014-06-19 WBIF IHCIP
Sigma-Aldrichx1000
H7915
Rabbit
IgG1

HeLa

【L】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
LC32011-08-29 WBIF IHCIP
MBLx50
rabbit
【PFA_70%EtOH】:○【PFA条件】3%PFA/PBS 37℃10min
Poly

Hela細胞
mouse LAMP12011-08-10 1D4BWBIF IHCIP
DSHBx100
1D4B
3%PFA (in PBS)
rat
ImEMにも使用可能
Applicable for ImEM
マウス小腸
mouse LAMP22011-08-12 WBIF IHCIP
DSHB
ABL93
rat
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
mouse LAMP22011-08-10 ABL93WBIF IHCIP
DSHBx100
ABL93
3%PFA (in PBS)
rat
ImEMには使えない(GAで失活)が、PFAでは一番よい
Not suitable for ImEM because antigenicity is lost by GA. Stains LAMP2 best of all lamp2 Abs by PFA fix.
マウス小腸
mouse LAMP22011-08-10 GL2A7WBIF IHCIP
DSHB
GL2A7
rat

human LAMP12011-08-10 H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA (in PBS)
mouse

Caco2細胞
human LAMP22011-08-10 H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA (in PBS)
mouse

Caco2細胞

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
p622011-08-29 WBIF IHCIP
Progenx1000x100
guinea pig
Poly

Hela細胞

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
SEL1L2018-07-26 WBIF IHCIP
Sigma1/10001-3ug
S3699
Rabbit

HCT116

【U】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Ubiquitylated Proteins2011-08-29 clone FKIIWBIF IHCIP
日本バイオテスト研究所 Nippon Bio-test Laboratoriesx1000
mouse
Mono, IgG1
Permeabilize before fixation
Hela細胞
Ubiquitin2011-08-29 MAB1510WBIF IHCIP
Chemiconx500
mouse
Mono, IgG1
Before WB, autoclave the membrane
Hela細胞
mouse ubiquilin2011-08-10 WBIF IHCIP
Calbiochem
662052
rabbit
p

試してない?
Not used
ubiquitin2011-09-27 FK2WBIF IHCIP
Enzo Life Science1:10001:2000
BML-PW8810-0500
mouse
m
Recognizes mono- and polyubiquitinylated conjugates. Species independent.
MEF, HeLa, A549, MCF7他
Ubiqutin2011-10-13 WBIF IHCIP
CHEMICONX200
MAB1510
mouse
PFA+Triton
Mono, IgG1

HT1080細胞
ubiquitin2011-08-10 WBIF IHCIP
Dako
Z0458
rabbit
p

試してない?
Not used